IL-31 anticorps

N° du produit ABIN6964030

Cet anticorps anti-IL-31 est un anticorps Souris Monoclonal détectant IL-31 dans Neut. Adapté pour Humain.

Aperçu rapide pour IL-31 anticorps (ABIN6964030)

Antigène: IL-31 (IL31) (Interleukin 31 (IL31))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-31 est non-conjugé

Application: Neutralization (Neut)

Clone: MT313

Détail du produit anti-IL-31 anticorps

Specificité: Native and recombinant human IL-31

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Recombinant human IL-31

Isotype: IgG1

Information d'application

Indications d'application: Neutralization of human IL-31 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: Neutralization of human IL-31 induced IL-6 production (see graph). To determine the neutralizing capacity and ND50, mixtures of human recombinant IL-31 (50 ng/ ml) and various concentrations of mAb MT313 were added to human aortic endothelial cells that had been pre-stimulated with recombinant human IFN-γ (10 ng/mL). Following overnight incubation, cell supernatants were quantified in IL-6 ELISA. Neutralization dose 50 (ND50): MT313 neutralized the effect of human IL-31 at a ND50value of 0.1-0.6 μg/mL when 50 ng/mL of human IL-31 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: supplied at 1 mg/mL in PBS free of preservatives

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Date de péremption: 18 months

Détails sur IL-31

Antigène: IL-31 (IL31) (Interleukin 31 (IL31))

Autre désignation: IL31

ID gène: 386653